What does Jesus mean by his reference to losing your life?

Mark 8:35 (ESV)

35 For whoever would save his lifewill lose it, but whoever loses his life for my sake and the gospel’s will save it.

Losing your life involves entrusting it to Jesus.1 Setting aside your own personal interests, obeying his commands even if it means suffering, shame, and death. When you lose your life in this way, you will save it. You will enjoy eternal spiritual life, everlasting communion with God when you die/when Christ returns in glory.